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Reviews
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By
Dr. Narotam Sharma 
,
Mr. Satish C Nautiyal 
,
Ms. Sarita Kumari
,
Ms. Khushboo Kumari
,
Mr. Vikram Singh
,
Mr. Krishan Kumar
,
Mr. Sandeep K Singh
,
Dr. Raj K Singh
,
Mr. Satish C Nautiyal
,
Ms. Sarita Kumari
,
Ms. Khushboo Kumari
,
Mr. Vikram Singh
,
Mr. Krishan Kumar
,
Mr. Sandeep K Singh
,
Dr. Raj K Singh
Submited on: 07 Mar 2013 11:39:09 AM GMT
Published on: 07 Mar 2013 01:18:22 PM GMT
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What are the main claims of the paper and how important are they?
I appreciate that the principles and characteristics of High Resolution Melting (HRM) analysis are very well summarized by authors.
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Are these claims novel? If not, please specify papers that weaken the claims to the originality of this one.
No, this paper introduces current HRM analytical methods.
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Are the claims properly placed in the context of the previous literature?
Yes
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Do the results support the claims? If not, what other evidence is required?
Yes
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If a protocol is provided, for example for a randomized controlled trial, are there any important deviations from it? If so, have the authors explained adequately why the deviations occurred?
NA
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Is the methodology valid? Does the paper offer enough details of its methodology that its experiments or its analyses could be reproduced?
NA
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Would any other experiments or additional information improve the paper? How much better would the paper be if this extra work was done, and how difficult would such work be to do, or to provide?
NA
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Is this paper outstanding in its discipline? (For example, would you like to see this work presented in a seminar at your hospital or university? Do you feel these results need to be incorporated in your next general lecture on the subject?) If yes, what makes it outstanding? If not, why not?
Yes
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Other Comments:
a. I feel like there are repetitive explanations about HR-1 and HRM, in the paragraph of “Innovations from instrument manufactures…..”. Please let us know about application of HRM analysis in SNP typing, clinical study and so on by citing concrete examples.
b. Instead of EvaGreen, LDGreen can be found in the ref. 5.
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Competing interests:
None
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Invited by the author to review this article? :
No -
Have you previously published on this or a similar topic?:
No
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References:
None -
Experience and credentials in the specific area of science:
Drug development
- How to cite: Yoshida T .Capsule High Resolution Melting (HRM) Analysis[Review of the article 'High Resolution Melt Curve Analysis- An Innovative Approach for Molecular Diagnosis ' by Singh R].WebmedCentral 2013;4(4):WMCRW002672
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The authors described "High Resolution Melting (HRM)" methods for SNP discovery, which is rapid and cost effective. The authors described a rapid and cost effective methods for SNP discovery, which is "High Resolution Melting (HRM)" Analysis. Short history, principle and comparison among other similar methods were shown.
No, this is one of traditional chemical methods for SNP discovery. Like as the authors described, some commercial products are available.
Yes, but their claims are weak because of their poor originality.
They did not show any experimental results. If they could show some examples of clinical implementation and their usability, their claims would sound powerful.
NA
Valid. The detailed principle of HRM was shown but its methodology is not enough detail to reproduce.
Yes. If clinical applications of HRM were shown, this manuscripts would be better.
No, it is simple summary.
About SNPs discovery method, next generation sequencing (NGS) technologies are very powerful and precise. HRM and other chemical methods are out-of-date. So they should clarify why they introduce this methods right now. I understand HRM is very simple and cheap, and there are some advantages of HRM analysis rather than NGS. The author should describe that by showing their experiences.
None
No
No
None
Yes, I performed SNPs discovery with Sanger capillary sequencers and Next Generation Sequencers.